RBGKEW1309 Long-Read Ash Genome Sequencing 2024
Buyer: Royal Botanic Gardens, Kew
- Published
- 22 August 2024
- Estimated value
- £150,000
- Place of performance
- UKI
- Procedure
- Open procedure
- Lots
- 1
- Notice number
- 026781-2024
- Reference
- ocds-h6vhtk-045914
- Official source
- Official source
Description
Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp. In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306. RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.
Awards
| Awarded to | Amount | Date |
|---|---|---|
| Novogene (UK) Company LTD | — | — |
Official publications
- FTS · 026781-2024 · 22 August 2024
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Frequently asked questions
- What is the estimated value of this tender?
- The estimated value published by the buyer is £150,000.
- Who is the buyer of this tender?
- The contracting authority is Royal Botanic Gardens, Kew (United Kingdom).
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