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Contract modification United Kingdom Services

Development and validation of a species-specific environmental DNA (eDNA) assay for American mink (Neovison vison)

Reference ocds-b5fd17-939d95c9-14f2-4657-9094-d1c1384288ca · Natural England

Contract modification. Estimated value: £25,000.

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Amounts and figures

£25,000
Estimated value

Description

The development of a dPCR assay for American mink (Neovison vison). 1. qPCR validation a. The successful contractor will evaluate four existing assays for American mink that target either the COI gene (Pugh 2022), cyt-b gene (Di Girolamo 2022) or ND2 gene (Takaba and others 2024) using the validation scale for single species eDNA assays (Thalinger and others 2021). b. Areas for assay improvement should be identified, and further in silico and in vitro testing conducted using qPCR to ensure at least one assay reaches Level 3 minimum on the validation scale (Thalinger and others 2021). The assay(s) should be able to achieve a R-squared value ≥0.990 and efficiency of 90-110%. c. If these validation criteria are not met, the successful contractor must design a new assay that does meet the validation criteria. d. A confidence assessment should be undertaken for the best-performing assay following the requirements set out in NECR359 "A framework for assessing confidence in environmental DNA qPCR assays and results" (K.J. Harper and others 2021). This framework (also known as COASTER) requires a standardised data input, as well as user-defined settings, and can be accessed throu…

Official publications

MediumReferenceDate
FTS 52e42ac0-db85-4545-a3db-0099eb914a42-780304 27 August 2024
Contracts Finder 52e42ac0-db85-4545-a3db-0099eb914a42-780304 27 August 2024

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Frequently asked questions

What is the deadline to bid for this tender?
The submission deadline is 5 September 2024, 12:00. Check the official source, as dates can be modified.
What is the estimated value of this tender?
The estimated value published by the buyer is £25,000.
Who is the buyer of this tender?
The contracting authority is Natural England (United Kingdom).
How can I bid for public tenders in the United Kingdom?
Bids are submitted through the official public procurement portal in the United Kingdom. El Vínculo helps you find tenders and analyse their documents; submission always happens at the official source.

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Data collected from official public procurement sources. Amounts as published by the buyer.