Voluntary ex ante transparency notice super-resolution microscope for 3-dimensional functional imaging (3D-SR-FI)
Buyer: Wageningen University & Research
- Published
- 17 September 2024
- Procedure
- Negotiated procedure
- Lots
- 1
- Notice number
- 00558452-2024
- Reference
- 2521c827-75b9-402a-b5f4-66b3a1075109
- Official source
- Official source
CPV codes
- 38515200Microscopios de fluorescencia
- 38510000Microscopios
Description
WUR needs a contract for the delivery of a microscope system, that is capable of 3D imaging of molecular and cellular functioning in living tissues, with an optical resolution below the diffraction limit. The general aim of node 2 of the NL-bioimaging consortium is to apply 3D functional imaging in complex living-cellular systems. 3D imaging of multicellular organisms like organoids or plants allows to obtain the landscape of molecular events and to reveal the mode of action of proteins in their natural habitat. Specifically at WUR, the focus of the 3D functional imaging node will be on ‘high resolution’, ‘endogenous expression’ and ‘FLIM/FCS’. The main requirement for the new system is that specimen can be imaged with a resolution beyond the diffraction limit (super-resolution microscopy or optical nanoscopy). The system should be able to image fluorescent proteins (FPs) expressed at endogenous levels in living plant tissues. Furthermore, the system should have functional imaging tools, such as fluorescence lifetime imaging microscopy (FLIM). Moreover, the conditions for super-resolution should not interfere with (multi-)cellular functioning. The main challenge for the system is…
Awards
| Awarded to | Amount | Date |
|---|---|---|
| Leica Microsystems B.V. | — | — |
Official publications
- TED · 00558452-2024 · 17 September 2024
- OJS · 181/2024 · 17 September 2024
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