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Closed European Union Supplies

Procurement of spectrofluorometer

Buyer: Aalborg Universitet

Published
4 September 2025
Submission deadline
22 September 2025, 22:00
Place of performance
DK050
Procedure
Other
Lots
1
Documents
1
Notice number
00578331-2025
Reference
22a9f2bd-196d-4bc0-a884-0659adc71e26
Official source
Official source

CPV codes

Description

Mutations in the archetypal Ca2+-sensor calmodulin (CaM) are associated with life-threat-ening cardiac arrhythmias in children. Yet, population wide sequencing studies now reveal carriers of CaM mutations who are seemingly healthy. This extreme segregation of phenotypes warrants a deeper understanding of the molecular impact of CaM mutations, to understand why some variants result in a pathogenic phenotype and other variants do not. Thus, the aim of this project is to determine which human Calmodulin mutations impair the ability to bind Ca2+-ions and decode intracellular Ca2+-signals to target proteins. We wish to upgrade our experimental platform with a new spectrofluorometer for measuring the Ca2+-affinity of CaM variants. Calcium binding to the CaM N- and C-lobe can be followed by intrinsic Phe and Tyr fluorescence, respectively. The change in fluorescence signal upon Ca2+-binding is small and demands high sensitivity and spectral specificity to be accurately and reliably measured. The optical configuration of the spectrofluorometer we seek needs to meet these requirements. The instrument should further be equipped with a fluorescence anisotropy module to enable characte…

Official publications

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Frequently asked questions

What is the deadline to bid for this tender?
The submission deadline is 22 September 2025, 22:00. Check the official source, as dates can be modified.
Who is the buyer of this tender?
The contracting authority is Aalborg Universitet (European Union).
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Data collected from official public procurement sources. Amounts as published by the buyer.