Microbial single cell genomics analyses

Published

14 April 2026

Place of performance

Maine

Procedure

neg-wo-call

Lots

1

Notice number

00254240-2026

Reference

4c391ebc-b4b8-456b-9a14-7297b705e275

Official source

Official source

CPV codes

• 73000000Servicios de investigación y desarrollo y servicios de consultoría conexos
• 73111000Servicios de laboratorio de investigación

Description

The Singe Cell Genomics Center (SCGC; https://scgc.bigelow.org) at the Bigelow Laboratory for Ocean Sciences is the only facility in the world capable of performing large-scale, integrated single-cell and single-viral-particle genome and phenome analyses on diverse environmental microbiomes. The processing of exceedingly small DNA quantities makes single cell genomics highly susceptible to DNA contamination and amplification biases. SCGC has developed techniques to monitor and minimize methodological artifacts at every step of its workflow. Cell sorting and DNA amplification are performed in a cleanroom, and all consumables are decontaminated using in-house methods. Control wells on each microplate are used to detect potential DNA contamination. To prevent index switching during DNA sequencing, multiplexed libraries contain dual 10 bp barcodes. Genome de novo assemblies are evaluated by diverse, in-house and external QC tools. The entire workflow is assessed for contamination and assembly errors using microbial benchmark cultures with diverse genome complexity and G+C content, indicating no non-target and undefined bases and average frequencies of mis-assemblies, indels, and mismat…

Awards

Frequently asked questions

Who is the buyer of this tender?

The contracting authority is Aalborg Universitet (European Union).